Can lipemia affect test results?

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How Does Lipemia Impact Laboratory Testing? Lipemia results from sample turbidity from accumulation of lipoprotein particles and can interfere with laboratory analysis by several mechanisms. First, lipemia can increase absorption of light and thereby decrease light transmittance used for spectrophotometric analysis.

How lipemia affect analyte measurement in clinical chemistry?

Lipemia interferes with chemistry tests by the following mechanisms: Light scattering: Results in falsely increased absorbance readings of some analytes, particularly those that are endpoint reactions that are not blanked, e.g. total bilirubin, resulting in high concentrations of bilirubin.

Which parameter is most likely affected by lipemia?

Q: What CBC parameters are affected when the specimen is lipemic? A: Lipemia in a blood specimen used for clinical evaluation can cause significant interference with obtaining accurate test values. Lipemia creates turbidity of a sample and is a result of the accumulation of lipid particles.

What CBC parameters are affected by lipemia?

When triglycerides exceed 300 mg/dL, turbidity occurs, directly affecting the ability to accurately obtain certain CBC parameters. Lipemia has been reported as a source of interference when measuring hemoglobin, white blood cells,4 platelets,5 and even lymphocytes.

Does lipemia affect AST?

Comparison of the results of native serum, serum blank, and diluted serum with ultracentrifuged serum. In moderate lipemia (700–1000 mg/dl), as shown in Table 3, only ALT, ALP, amylase, AST, bilirubin, and uric acid are not influenced by lipemia.

Does lipemia affect triglycerides?

Lipemia can be detected visually if the concentration of triglycerides in patient sample is over 3.4 mmol/L (32). In the full blood samples, visual detection is very hard and can be observed at much higher concentration of triglycerides (over 11.3 mmol/L) (32).

How can lipemic specimens affect CRP results?

This effect could be explained, since lipemia produces an increase in Cp values due to light scattering, as occurs with hemolysis, but when the interference is great enough to raise the absorbance above the range of linearity, the effect is the opposite. Lipemia also modified CRP levels significantly.

Does lipemia affect bun?

BUN was the only analyte that was affected by gross lipemia at triglyceride concentrations of 2100 mg/dL with ordinal values of “+++++” flag level. Visual inspection results for hemolysis and lipemia showed good agreement between three technologists and were consistent with the corresponding ordinal values.

How does hemolysis affect chemistry tests?

Certain lab tests can be affected and the reported results will be inaccurate. It falsely decreases values such as RBC’s, HCT, and aPTT. It can also falsely elevate potassium, ammonia, magnesium, phosphorus, AST, ALT, LDH and PT.

Which RBC indices are affected by lipemia?

A. Lipemia interferes with the accurate determination of hemoglobin, or Hb, by spectroscopy on most hematology analyzers, but it does not generally interfere with determinations (especially impedance based) of red blood cell count, white blood cell count, and platelet count.

Is lipase affected by hemolysis?

Hemolysis may falsely increase the following analytes: AST, alanine transaminase (ALT), LDH, total bilirubin, glucose, calcium, phosphorus, total protein, albumin, magnesium, amylase, lipase, creatine kinase (CK), iron, hemoglobin, and mean corpuscular hemoglobin concentration (MCHC).

Which of the following tests would most likely be included in a routine lipid profile?

Which of the following tests would most likely be included in a routine lipid profile? D. A “routine” lipid profile would most likely consist of the measurement of total cholesterol, triglyceride, HDL cholesterol and LDL cholesterol.

How does lipemia affect electrolytes?

Considering 0-350 mg% of triglyceride as the reference, electrolytes concentration mostly decreased over increasing lipemia. Beyond triglyceride concentration of 650mg%, this decline in electrolytes concentration was statistically significantly for samples in all subgroups.

What on the CBC will falsely elevate the Wbcs?

Falsely elevated WBC values may be due to aggregated platelet clumps or incomplete rbc lysis.

Are triglycerides affected by hemolysis?

We conclude that hemolysis causes transient hyperlipidemia, either directly by red cell destruction or indirectly by inducing intravascular coagulation, and possibly due to both increased triglyceride synthesis and decreased catabolism.

What chemistry analytes are affected by hemolysis?

Two analytes greatly impacted by hemolysis are potassium and lactate dehydrogenase, in which their concentrations in erythrocytes are more than 20 times and 150 times higher than it is in serum, respectively.

Are lipids affected by hemolysis?

Increased Lipid Concentration Is Associated with Increased Hemolysis.

What causes high lipemia?

The most common likely causes of elevated lipemic index were lipid-containing intravenous medications (fat emulsion for parenteral nutrition; propofol) and diabetes mellitus (mainly type 2).

What can cause a false high triglycerides?

Other Factors Drinking alcohol and smoking prior to testing can cause false results. Recent exercise and fluctuating hormone levels may also affect triglyceride readings, making them falsely high or low.

Is sodium affected by hemolysis?

As previously reported, in vitro hemolysis is known to negatively interfere with sodium due to a diluting effect (13,14), as the intracellular concentration of sodium is significantly lower than the concentration in serum or plasma.

What is Lipaemic blood sample?

Lipaemic samples are caused by an excess of lipoproteins in the blood, creating a milky/turbid appearance that interferes with multiple biochemical tests and can even cause haemolysis of red blood cells. A severely lipaemic sample (red arrow).

What can cause false positive CRP results?

Lifestyle: People who smoke, have obesity, or do little exercise may have higher-than-normal CRP levels. Minor injuries or infection: These conditions may temporarily raise CRP levels and conceal other potential conditions, such as diabetes or IBD.

Is Phos affected by hemolysis?

Moderate hemolysis significantly increased plasma phosphorus levels. Marked hemolysis significantly increased plasma values of potassium, phosphorus, total protein, and aspartate aminotransferase.

Is chloride affected by hemolysis?

Even at the greatest hemolysis degree, HI=(4+), no interference was detected for calcium, chloride, creatinine, C-reactive protein (CRP), glucose and sodium.

What test is most affected by hemolysis?

Lactate dehydrogenase appeared to be most sensitive to hemolysis; the increase of č 1000 U of lactate dehydrogenase per liter resulted in a 4.5-fold higher enzyme activity at 4.5 g of hemoglobin per liter of plasma than at 0.27 g/L.

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