To prevent corrosion in your biosafety cabinet, use a squeeze bottle of 10% bleach (rather than spray) and follow it with a 70% ethanol rinse.
When should the work surface of a biosafety cabinet be disinfected?
Once the cabinet is empty, you should allow 20-30 minutes to thoroughly decontaminate all interior work surfaces, making sure to clean the back and side of the cabinet, and the interior of the glass.
Can you use chemicals in biological safety cabinets?
Volatile and hazardous chemicals are not permitted in BSCs unless they are specifically designed for that purpose and are properly vented, and then only small or minute amounts are allowed. All BSCs use HEPA filters to treat exhaust air.
How do you decontaminate a hood?
Wipe down all surfaces in the hood with soapy water and then 70% ethanol starting from the back and working your way forwards. Again, products that contain bleach are not ideal here as you do not want to damage the hood. Don’t forget the sash and the outside of the hood.
What do you use for decontamination?
- Hypochlorite (Bleach)
- Chlorine Dioxide.
- Hydrogen Peroxide & Peracetic Acid.
- Iodophor Disinfectant (Wescodyne)
- Quaternary Ammonium Compounds (Quats)
What is used for decontamination of a large laboratory space?
Paraformaldehyde and formaldehyde are often used to decontaminate large pieces of laboratory equipment, such as biosafety cabinets (but only by professionals!). Paraformaldehyde/formaldehyde will inactivate vegetative bacteria, fungi, lipid and non-lipid viruses, HBV, TB, Coxiella burnetii, and bacterial spores.
Do and don’ts of biosafety cabinet?
- Never have the ultraviolet light on when working at the cabinet.
- Avoid disrupting airflow:
- Bunsen burners, other continuous flame devices, or flammable gases are prohibited in biosafety cabinets.
- Beware of fire hazards associated with vaporized ethanol and isopropanol disinfectants.
What is decontamination process?
Decontamination is a combination of processes that removes or destroys contamination so that infectious agents or other contaminants cannot reach a susceptible site in sufficient quantities to initiate infection, or other harmful response.
How often does a biosafety cabinet need to be certified?
BSCs must be certified when installed, whenever they are moved and at least annually [29 CFR 1910.1030(e)(2) (iii)(B)]. Employers should ensure that a risk assessment has been completed and approved for the work to be conducted and to identify the class and type of BSC needed for the operation or procedure.
Is biosafety cabinet sterile?
The Class II Type A2 biosafety cabinet is the most common cabinet on campus. It uses a curtain of air and HEPA filters to provide both containment and a sterile environment.
What is HEPA filter in biosafety cabinet?
An essential component in any clean bench or biosafety cabinet is the high efficiency particulate air filter, commonly called a HEPA filter. The HEPA filter removes particulates (generally called aerosols) such as micro-organisms, from the air. The HEPA filter does not remove vapors or gases.
Which cabinet would you use when working with biological samples with toxic and volatile chemicals?
Biological safety cabinets (or biosafety cabinets or BSCs) utilize HEPA filters to provide environmental, personnel and product protection from hazardous particulates like bacteria and viruses and typically recirculate filtered air back to the lab (depending on Type).
How do you sterilize a laminar hood?
Swab the bottom edges and lower back corners. Then clean the work surface, back to front, left to right. Repeat this cleaning process using a sterilizing agent such as USP grade sterile 70% isopropanol.
What type of cleaning agent should be used to clean a laminar flow hood?
Whether you work with a vertical or horizontal flow hood, you can adjust the procedure as needed. A good cleaning agent to use is USP grade sterile 70% isopropanol.
What is the correct order of cleaning the flow hood?
Clean the ceiling of the unit first. Clean the back wall (of a vertical laminar flow hood) next, going from top to bottom. Clean the sides (swiping from top to bottom in overlapping lines). The work surface should be the last surface cleaned, beginning in the back and ending at the front of the unit.
What are the 3 steps of decontamination?
There are three levels of decontamination, general cleaning, disinfection and sterilisation. Equipment used in health care may be designated as single use, single patient use or reusable multi-patient use.
What are the 4 stages of decontamination?
- pre-sterilisation cleaning.
What are the three types of decontamination?
Decontamination methods either (1) physically remove contaminants, (2) inactivate contaminants by chemical detoxification or disinfection/sterilization, or (3) remove contaminants by a combination of both physical and chemical means.
How do you decontaminate laboratory equipment?
Following use of a chlorine-based disinfectant, wipe down all surfaces (including underneath the workspace and grill) with water, followed by 70% ethanol or 70% isopropanol (v/v, diluted in water) to remove any residue left by the disinfectant. This will help prevent subsequent corrosion of cabinet surfaces.
What are the two categories of biological decontamination?
Decontamination falls into two categories: disinfection and sterilization.
How do you decontaminate a lab refrigerator?
If biological agents have spilled or leaked, clean with a 10% bleach solution (1 part bleach to 9 parts water). Physical Facilities personnel can then be contacted. Combination of chemicals/drugs and biological agents: Remove all items and defrost.
What are the 3 best practices that make the biological safety cabinet a safe place for work?
- Disinfect work surfaces before and after working to minimize or eliminate surface contamination.
- Ensure that materials brought into the hood are either disinfected or sterilized.
What are the guidelines to be followed while working at the biosafety cabinet?
- Know Your Airflow.
- Work at the Proper Sash Level.
- Never Block the Airflow Grill.
- Minimize Movement.
- Reduce Splatter.
- Know Your Work Area.
What should I do when working in BSC?
Working in the BSC: Ensure the vertical sliding sash at the correct height. Allow only one BSCs operator at a time. Sit at the BSC with armpits level with the bottom of the sash. Perform all operations at least 4 inches from the front grille on the work surface.
What is the most important step in the decontamination process?
“The first and most important step in decontamination is thorough cleaning and rinsing,” says the Association for the Advancement of Medical Instrumentation’s Comprehensive Guide to Steam Sterilization (ANSI/AAMI ST79:2006).