- C1 is the concentration of the starting solution.
- V1 is the volume of the starting solution.
- C2 is the concentration of the final solution.
- V2 is the volume of the final solution.
Table of Contents
How do you calculate a dilution factor in chemistry?

How do you calculate the dilution of a liquid?
Calculate appropriate v/v dilution using the formula C1V1 = C2V2 where C represents the concentration of the solute, and V represents volume in milliliters or ml. An example would be combining 95 percent ethanol with water to mix 100 ml of 70 percent ethanol. The calculation is 95% X V1 = 70% X 100ml.
How do you calculate dilution ratio mL?
Multiply the final desired volume by the dilution factor to determine the needed volume of the stock solution. In our example, 30 mL x 1 รท 20 = 1.5 mL of stock solution. Subtract this figure from the final desired volume to calculate the volume of diluent required–for example, 30 mL – 1.5 mL = 28.5 mL.
What does a 1 in 10 dilution mean?
For example, to make a 1:10 dilution of a 1M NaCl solution, you would mix one “part” of the 1M solution with nine “parts” of solvent (probably water), for a total of ten “parts.” Therefore, 1:10 dilution means 1 part + 9 parts of water (or other diluent).
What is a 1 to 20 dilution?
A 1:20 dilution implies that you take 1 part of stock solution and add 19 parts of water to get a total volume of diluted solution equal to 20 times that of the stock solution.
What is a 1 in 50 dilution?
Explanation: If you want to make a 1/50 dilution you add 1 volume part of the one to 49 parts of the other, to make up 50 parts in all.
How do you solve dilution problems?

How do you dilute a 40% solution to 10 %?
So, if you add 7.2 oz of solvent to your 2-oz , 40% solution will get a 10% solution that has the total volume of 9,2 oz .
How do you calculate the concentration of a dilute solution?
Calculate concentration of solution after dilution: c2 = (c1V1) รท V. Calculate the new concentration in mol L-1 (molarity) if enough water is added to 100.00 mL of 0.25 mol L-1 sodium chloride solution to make up 1.5 L.
How do you do a 1 to 10 dilution?
When products come full strength, you can dilute them to suit your cleaning needs to get more bang for buck and make the products last longer. For example, a 10:1 ratio means you mix 10 parts water to 1 part chemical. The amount of each liquid changes depending on the ratio used, and the size of the container.
What is a 10% dilution?
The 10 represents the total size of the final sample. For example, a sample size of 1 ml is added to 9 ml of diluent to equal a total of 10 ml. Example: 1:10 dilution – if the concentration is 1,000 CFU, a one log dilution will drop the concentration to 100 CFU. Initial sample.
How do you do a 1 to 3 dilution?
The diluted liquid needs to be thoroughly mixed to achieve true dilution. If you have a 1:3 dilution, i.e. a 1:3 dilution ratio, this means that you add 1 unit volume of solute (e.g., concentrate) to 3 unit volumes of the solvent (e.g., water), which will give a total of 4 units of volume.
How do you make a 1 to 4 dilution?
A 1:4 dilution ratio means that a simple dilution contains one part concentrated solution or solute and four parts of the solvent, which is usually water. For example, frozen juice that requires one can of frozen juice plus four cans of water is a 1:4 simple dilution.
How do you make a 10X dilution?
Using this terminology, a “10X” stock might be diluted by adding 100 mL of the stock to 900 mL to produce a “1X” working solution. In this lab we will be working with a stock stock solution of a fluorescent compound called fluorescein.
How do you make a 2x dilution?
Dilution by Adding Solvent to an End Volume: Consider the case of the alcohol above. Since 50mL of the alcohol was diluted to a final volume of 100mL, we say the alcohol was diluted “two times,” “twice,” or “2x.” For the acid, 20mL was diluted to 500mL, so it would be described as being diluted 25 times (500/20 = 25).
What is a 5% dilution?
How much initial sample and diluent should you use? Answer: 1:5 dilution = 1/5 dilution = 1 part sample and 4 parts diluent in a total of 5 parts. If you need 10 ml, final volume, then you need 1/5 of 10 ml = 2 ml sample. To bring this 2 ml sample up to a total volume of 10 ml, you must add 10 ml – 2 ml = 8 ml diluent.
What is a 20x dilution?
A solution 20 times more concentrated would be denoted as 20x and would require a 1:20 dilution to restore the typical working concentration. Example: A 1x solution of a compound has a molar concentration of 0.05 M for its typical use in a lab procedure.
How do you calculate a 50x dilution?

How do you make a 1 300 dilution?
Example 1: To dilute a streptavidin solution 1:300 Mix 1 unit volume of streptavidin solution with 299 volumes of solvent medium. In some instances, solutions are expressed in terms of “X,” an indicator of relative solute concentration.
How do you make a 1 80 dilution?
So, to make a 1:80 dilution for a 1-mL sample, you add enough water to get the final volume to 80 mL โ in this example, you would add 79 mL of water to the 1-mL sample.
How do you make a 20% solution?
You would need to put in 80 mL of water and then add 20 mL of acid to make a 20% solution.
How do you calculate concentration using dilution factor?
A general rule to use in calculating the concentration of solutions in a series is to multiply the original concentration by the first dilution factor, this by the second dilution factor, this by the third dilution factor, and so on until the final concentration is known. Example: A 5M solution of HCl is diluted 1/5.
What is a 7 1 dilution?
The minimum dilution rate, according to the label, is 7:1. This means that your final spray mix should be 7 parts water and 1 part concentrate, or stated another way, there should be 7 times more water than concentrate in your final mix. 1 gallon of water is 128 ounces.
What is dilution method?
Dilution Method To obtain the desired concentration, a simple dilution is one in which a unit volume of a liquid material of interest is blended with an adequate volume of a solvent liquid. The total number of unit volumes in which the material will be dissolved is the dilution factor.