How do you clear a lipemic sample?

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High-speed centrifugation (10,000×g for 15 minutes) can be used instead of ultracentrifugation to remove lipemia in serum/plasma samples.

How does lipemia affect chemistry results?

How Does Lipemia Impact Laboratory Testing? Lipemia results from sample turbidity from accumulation of lipoprotein particles and can interfere with laboratory analysis by several mechanisms. First, lipemia can increase absorption of light and thereby decrease light transmittance used for spectrophotometric analysis.

How lipemia affect analyte measurement in clinical chemistry?

Lipemia interferes with chemistry tests by the following mechanisms: Light scattering: Results in falsely increased absorbance readings of some analytes, particularly those that are endpoint reactions that are not blanked, e.g. total bilirubin, resulting in high concentrations of bilirubin.

Is lipemic serum acceptable for chemistry testing?

As a common interferer in clinical chemistry, lipemic specimens could be a source of significant analytical errors. Ultracentrifugation has been by far the only reliable, but an unavailable and expensive, method to eliminate the lipemic effect.

Which chemistry analytes are most affected by lipemia?

Conclusion: Lipemia causes clinically significant interferences for phosphorus, creatinine, total protein and calcium measurement and those interferences could be effectively removed by ultracentrifugation.

Which parameter is most likely affected by lipemia?

Q: What CBC parameters are affected when the specimen is lipemic? A: Lipemia in a blood specimen used for clinical evaluation can cause significant interference with obtaining accurate test values. Lipemia creates turbidity of a sample and is a result of the accumulation of lipid particles.

Why does lipemia in specimen cause an inaccurate hemoglobin value?

Lipemic specimens contain high levels of triglycerides consisting of chylomicrons and very low-density lipoprotein particles, which in turn cause turbidity. This turbidity interferes with light scatter and the absorption of light, resulting in a false increase of hemoglobin determinations.

How does hemolysis affect chemistry tests?

Certain lab tests can be affected and the reported results will be inaccurate. It falsely decreases values such as RBC’s, HCT, and aPTT. It can also falsely elevate potassium, ammonia, magnesium, phosphorus, AST, ALT, LDH and PT.

What does lipemic serum indicate?

Lipemia is a measure of serum transparency. High levels of blood lipids, mostly triglycerides, increase serum turbidity. The lipemia result is expressed in “number of plus signs,” ranging from negative to four pluses (++++).

How do you check for clots hemolysis and lipemia on a sample?

Sample collection and handling procedures Collect blood into a plain, red top tube. Allow blood to clot, and then centrifuged to separate serum. Transfer serum to a new plain, red top tube. Samples with marked hemolysis or lipemia should be re-drawn before sample submission.

What chemistry analytes are affected by hemolysis?

Two analytes greatly impacted by hemolysis are potassium and lactate dehydrogenase, in which their concentrations in erythrocytes are more than 20 times and 150 times higher than it is in serum, respectively.

What causes a lipemic sample?

After hemolysis, lipemia is the most frequent endogenous interference that can influence results of various laboratory methods by several mechanisms. The most common preanalytical cause of lipemic samples is inadequate time of blood sampling after the meal or parenteral administration of synthetic lipid emulsions.

How do you reduce lipemia?

Aerobic exercise conducted 11–20 hours before the test meal appears to produce the greatest magnitude of reduction in postprandial lipemia compared to aerobic exercise conducted 30 minutes to 4 hours before a test meal [65, 66].

How do you prevent lipemia?

One way to avoid grossly lipemic samples is to ask that patients fast for 12 hours before sample collection. If this is impractical, a mechanical-based means of clot detection should be available when samples are grossly lipemic.

How can lipemic plasma be prevented?

Please avoid food with high fat content before blood donation. If a blood donor consumes food with high fat content such as oily foods which are fried or deep fried before blood donation it can lead to a transient rise in the triglycerides or cholesterol levels resulting in lipemic blood.

Which RBC indices are affected by lipemia?

A. Lipemia interferes with the accurate determination of hemoglobin, or Hb, by spectroscopy on most hematology analyzers, but it does not generally interfere with determinations (especially impedance based) of red blood cell count, white blood cell count, and platelet count.

What causes high lipemia?

The most common likely causes of elevated lipemic index were lipid-containing intravenous medications (fat emulsion for parenteral nutrition; propofol) and diabetes mellitus (mainly type 2).

Are lipids affected by hemolysis?

Increased Lipid Concentration Is Associated with Increased Hemolysis.

Which test is most affected by hemolysis?

Lactate dehydrogenase appeared to be most sensitive to hemolysis; the increase of č 1000 U of lactate dehydrogenase per liter resulted in a 4.5-fold higher enzyme activity at 4.5 g of hemoglobin per liter of plasma than at 0.27 g/L.

What tests are not affected by hemolysis?

Analytes that were not affected at even the highest level of hemolysis include calcium, chloride, creatinine, C-reactive protein (CRP), glucose and sodium.

What does Lipaemic mean?

: the presence of an excess of fats or lipids in the blood specifically : hypercholesterolemia.

Does lipemia affect coagulation?

Introduction: Lipemia in samples can cause analytical errors in coagulation tests using photometric assays.

How does lipemia affect electrolytes?

Considering 0-350 mg% of triglyceride as the reference, electrolytes concentration mostly decreased over increasing lipemia. Beyond triglyceride concentration of 650mg%, this decline in electrolytes concentration was statistically significantly for samples in all subgroups.

Why is EDTA the best anticoagulant?

It inhibits clotting by removing or chelating calcium from the blood. EDTA most important advantage is that it does not distort blood cells, making it ideal for the most hematological tests.

Does lipemia affect triglycerides?

Lipemia can be detected visually if the concentration of triglycerides in patient sample is over 3.4 mmol/L (32). In the full blood samples, visual detection is very hard and can be observed at much higher concentration of triglycerides (over 11.3 mmol/L) (32).

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