Open the pipette, immerse the parts in a detergent solution, such as Deconex® 12 Basic. Rinse well with distilled water and allow to dry. DNA can be eliminated by immersing pipette parts in at least 3% (w/v) sodium hypochlorite for at least 15 minutes (2,3). Rinse well with distilled water and allow to dry.
Table of Contents
How do you take care of a pipette?
- Decontaminate the outside of the pipette.
- Inspect the general appearance of the pipette.
- Disassemble and clean the pipette.
- Replace the o-ring(s) and seal(s)
- Lubricate (if applicable)
- Re-assemble the pipette and check functionality.
How do you use a pipette in biology?
How do you clean and condition a pipette?
For organic solvents, allow the substance to evaporate on its own or immerse the part in a detergent and then air dry. For radioactive substances, place the pipette in a solution like Decon and then rinse and air dry. For proteins, rinse the contaminated parts with a detergent, rinse, and air dry.
What is used to clean pipette?
Open the pipette, immerse the parts in a detergent solution, such as Deconex® 12 Basic. Rinse well with distilled water and allow to dry. DNA can be eliminated by immersing pipette parts in at least 3% (w/v) sodium hypochlorite for at least 15 minutes (2,3). Rinse well with distilled water and allow to dry.
How do you take care of a lab pipette?
- Store pipettes vertically to avoid contamination.
- Store pipettes dialed to highest volume setting.
- Store away from heat and moisture.
- Pipette tips should be removed prior to storage.
- Keep pipettes clean and free of contaminants.
How do you deep clean a pipette?
Immerse your pipette parts in at least 3% (w/v) sodium hypochlorite for at least 15 minutes. Rinse with distilled water. Air dry as above. You can also use UV light to further reduce DNA contamination.
How do you decontaminate pipettes with bleach?
How and when to decontaminate. Generously spray workstations/equipment/pipettes with 10% bleach, then let it sit for 15-30 minutes (coffee break time!). Then wipe up the bleach and follow up with a water rinse and wipe — bleach is corrosive, so it will damage materials if residue is not removed by rinsing with water.
What actions should you take when using a pipette to avoid cross contamination?
Prevention: Keep the pipette vertical when pipetting in order to prevent liquid from running into the pipette body. Release the push button slowly. To avoid aerosol contamination, use filter tips or use a positive displacement pipette and tips.
What is pipette and its function?
A pipette (sometimes spelled as pipett) is a laboratory tool commonly used in chemistry, biology and medicine to transport a measured volume of liquid, often as a media dispenser.
How does a pipette work?
In the air cushion principle, an air cushion separates the liquid in the tip from the pis- ton inside the pipette. The piston moves the air cushion and the liquid is thus taken up into the pipette tip or dispensed out of it. The air cushion thus works like an elas- tic spring, to which the liquid sticks.
How do you use a pipette accurately?
Put the pipette—held at 10–45 degrees—against the wall of the receiving chamber, and smoothly depress the plunger to the first stop. Wait one second and then depress the plunger to the second stop. Slide the tip up the vessel wall to remove the pipette. Allow the plunger to return to its rest position.
How do you clean a pipette bottle?
- Step 1: Clean & Soak. Ensure your bottle is empty.
- Step 2: Rinse, Repeat.
- Step 3: Boil for Ten Minutes.
- Step 4: Rinse in 70% Isopropyl Alcohol.
- Step 5: Air Dry.
How do you clean a volumetric pipette?
Draw distilled water into the pipet and rinse out the soapy water. Rinse several times to remove all soap residue.
Why must the pipette be rinsed with the sample solution?
Rinsing with solution to be used ensures removal of any moisture adhering to glass. To contain the reaction mixture. Rinsing with the solution to be added from the pipette would increase the moles of substance used in the reaction, so the titre volume would be greater than it should be.
How often should you clean a pipette?
Cleaning the outer surface of your pipette is quick and easy, and should be part of your daily routine. Wiping them with a lint-free cloth lightly soaked with 70 % ethanol should be sufficient, but do check the chemical compatibility of the pipettes beforehand.
Can you wash pipette tips?
Yes. Conical tubes and even pipette tips can be washed and/or autoclaved and reused. Pipette tip boxes can be repurposed to hold other lab supplies or as containers for Western blots.
How do you dry pipettes?
Lay the pipettes on a clean towel so they dry. Spread out a soft towel on a flat surface. Lay your pipettes and other pieces out on the towel overnight so they have plenty of time to dry completely. Once the pipettes are dry, they’re ready to be reused.
How do you make a pipette better?
- Pre-wet the pipette tip.
- Immerse the tip to the proper depth during aspiration.
- Pause consistently after aspiration.
- Use consistent plunger pressure and speed.
- Pull the pipette straight out.
- Examine the tip BEFORE dispensing a sample.
- Examine the tip AFTER dispensing a sample.
- Use standard mode pipetting.
How do you maintain pipetting accuracy?
- Pre-rinse your new pipette tips before aspirating a sample for delivery.
- Ensure the tips immersion depth is correct.
- Ensure the micropipette is upright during pipetting.
- Apply consistent plunger pressure and speed.
- Good micropipette.
How do you test a pipette?
The most common way to check your pipette accuracy is by weighing water. The density of water is 1 g/mL. This means that every microliter (µL) should weigh exactly 0.001 g using a high-precision balance.
How do you sterilize glass pipettes?
Dry heat sterilize at 160°C (320°F) for at least 2 hours. If pipettes are steam sterilized, then dry thoroughly in a drying oven before use.
Does bleach remove DNA?
Ancient DNA (aDNA) researchers and forensic scientists have employed a number of techniques to minimize such contamination. One such technique is the use of bleach (sodium hypochlorite–NaOCl) to “destroy” contaminating DNA.
Can you UV a pipette?
UV-disinfection of pipettes: The Eppendorf Pipettes may be temporarily subjected to UV light under a sterile bench (see the operating manual for pipettes of other manufacturers). We recommend the use of a Hg-lamp, 254 nm, 30 W. The distance between lamp and pipette should be approx. 60 cm.
Does chlorine denature DNA?
Yes, if you know what you’re doing. Knox and Sollecito were on the right track: Bleach contains sodium hypochlorite, an extremely corrosive chemical that can break the hydrogen bonds between DNA base pairs and thus degrade or “denature” a DNA sample.