How is photosynthesis measured a level biology?

Measuring photosynthesis via the uptake of carbon dioxide Using an IRGA – Uptake of CO2 can be measured with the means of an IRGA (Infra-Red Gas Analyser) which can compare the CO2 concentration in gas passing into a chamber surrounding a leaf/plant and the CO2 leaving the chamber.

What is DCPIP used for in photosynthesis experiments?

DCPIP can be used to measure the rate of photosynthesis. It is part of the Hill reagents family. When exposed to light in a photosynthetic system, the dye is decolorised by chemical reduction.

How do you investigate photosynthesis?

Investigating photosynthesis. The effect of light intensity on photosynthesis can be investigated in water plants. Use Cabomba or Elodea, which are sold in aquarium shops. The plants will release bubbles of oxygen – a product of photosynthesis – which can be counted.

How do you isolate chloroplasts from leaves a level biology?

In chloroplast isolation method, the cell wall is broken mechanically using a blender or homogenizer. Then the unbroken leaf tissue and the cellular debris are removed by filtration. The chloroplasts are collected by centrifugation using a percoll gradient.

How is photosynthesis measured experiment?

What is the dependent variable in photosynthesis experiment?

Independent variable: the light intensity (how close the light is). Dependant variable: the number of oxygen bubbles given off (the rate of photosynthesis).

Why does DPIP change from blue to colorless?

When the dye is oxidized, it is blue. When reduced, however, it turns colorless. Since DPIP replaces NADPH in the light reactions, it will turn from blue to colorless when reduced during photosynthesis.

Why does DCPIP turn Colourless?

Reduced DCPIP is colourless. The loss of colour in the DCPIP is due to reducing agent produced by light-dependent reactions in the extracted chloroplasts.

What is Hill reaction in photosynthesis?

The Hill reaction is the portion of the light reactions in which electrons from water are transferred to an electron acceptor, reducing the acceptor. This reaction was first observed by Robert Hill in 1937 and it was he who demonstrated that isolated chloroplasts can produce O₂ in the absence of CO2.

How do you test a leaf for photosynthesis?

When you put iodine on the leaves, one of them will turn blue-black and the other will be a reddish-brown. Iodine is an indicator that turns blue-black in the presence of starch. The leaf that was in the light turns blue-black, which demonstrates that the leaf has been performing photosynthesis and producing starch.

How does CO2 affect the rate of photosynthesis experiment?

The null hypothesis was that the concentration of carbon dioxide will have no effect on the rate of photosynthesis. The results of the experiment supported the hypothesis. So, the experiment suggests that there is a direct relationship between CO2 concentration and the rate of photosynthesis.

How do you test for oxygen in photosynthesis?

How do you extract chlorophyll from leaves?

Dump the ground up leaf into a jar and soak with 15-25 milliliters of isopropyl alcohol for 15 minutes. Set up the funnel on top of another glass jar. Place a coffee filter over the top of the funnel and push it down into the funnel. Pour the extract from the first jar into the coffee filter.

Why are the major veins removed from leaves before isolation of chloroplasts?

We have to remove the large veins because these parts do not have much DNA to be extracted and grinding process will be hampered because it is very solid and stubborn, also it contains a large quantities of contaminants (salts and phenols).

How would you isolate the organelle responsible for photosynthesis?

Pigments are separated according to differences in their relative solubilities. In order to extract these pigments from the thylakoid membranes of the chloroplasts, the organelles in which photosynthesis occurs, fresh, ground or torn leaves (preferably spinach) may be soaked in acetone or concentrated alcohol.

Why water plant is used in photosynthesis experiment?

Plants produce oxygen which is invisible and odorless, so the experiment is usually carried out with water plants; the bubbles of oxygen produced are observed.

How does water affect the rate of photosynthesis experiment?

Water affects the rate of photosynthesis indirectly. When the water supply is limited then stomatal closure affects the rate of photosynthesis. Water stress causes the stomata to close and thereby reduces carbon dioxide availability.

Do different colors of light affect the rate of photosynthesis experiment?

The lights higher on the light spectrum, like blue, would have the fastest rate of photosynthesis because the energy is the highest. The green light would have the slowest rate because the chlorophyll inside of the leaves reflects green light, so it would not be absorbed as much as the other colors.

What is a good experiment for photosynthesis?

Use a measuring spoon to scoop out 1/8 teaspoon of baking soda, and fill your measuring cup with 300 milliliters of water. Mix the baking soda into the water to create your bicarbonate solution. When the baking soda dissolves in the water, it releases carbon dioxide. This will be used by the spinach for photosynthesis.

What factors affect the rate of photosynthesis?

The main factors affecting rate of photosynthesis are light intensity, carbon dioxide concentration and temperature.

How does temperature affect photosynthesis experiment?

If an experiment was conducted to test the rate of photosynthesis in room, cold, and warm temperature environments, then the leaves in the warm temperature environment will photosynthesize completely the fastest. In order for photosynthesis to occur, a plant needs a source of light and the most common one is the sun.

Why is DCPIP used in the Hill reaction?

DCPIP is blue when oxidised (at pH 7.0) and colourless when reduced, so it is possible to monitor the loss of blue colour as an indication that DCPIP has accepted electrons. It can be used to participate in, and monitor, redox reactions.

When DCPIP is blue What happens to the absorbance?

2,6-Dichlorophenolindophenol (DCPIP, DCIP or DPIP) is a chemical compound used as a redox dye. When oxidized, DCPIP is blue with a maximal absorption at 600 nm; when reduced, DCPIP is colorless.

What happens when you boil chloroplasts?

Boiling the chloroplast would rupture and destroy the chloroplast, therefore ceasing the process of photosynthesis. If unboiled chloroplast was in the cuvette, but light was absent, photosynthesis would not occur. Light is important in the process of photosynthesis.

What happens when DCPIP is added to vitamin C?

DCPIP is a dye. It is blue color when in oxidizing form and colorless in reduction form. When DCPIP is added into vitamin C solution, the vitamin C reduces the dye, then, decolorizes the dye. Therefore, the decolorization of DCPIP indicates the presence of vitamin C.

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