As a common interferer in clinical chemistry, lipemic specimens could be a source of significant analytical errors. Ultracentrifugation has been by far the only reliable, but an unavailable and expensive, method to eliminate the lipemic effect.
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How does lipemia affect chemistry results?
How Does Lipemia Impact Laboratory Testing? Lipemia results from sample turbidity from accumulation of lipoprotein particles and can interfere with laboratory analysis by several mechanisms. First, lipemia can increase absorption of light and thereby decrease light transmittance used for spectrophotometric analysis.
How lipemia affect analyte measurement in clinical chemistry?
Lipemia interferes with chemistry tests by the following mechanisms: Light scattering: Results in falsely increased absorbance readings of some analytes, particularly those that are endpoint reactions that are not blanked, e.g. total bilirubin, resulting in high concentrations of bilirubin.
Which chemistry analytes are most affected by lipemia?
Conclusion: Lipemia causes clinically significant interferences for phosphorus, creatinine, total protein and calcium measurement and those interferences could be effectively removed by ultracentrifugation.
How do you clear a lipemic sample?
High-speed centrifugation (10,000×g for 15 minutes) can be used instead of ultracentrifugation to remove lipemia in serum/plasma samples.
Which parameter is most likely affected by lipemia?
Q: What CBC parameters are affected when the specimen is lipemic? A: Lipemia in a blood specimen used for clinical evaluation can cause significant interference with obtaining accurate test values. Lipemia creates turbidity of a sample and is a result of the accumulation of lipid particles.
Why does lipemia in specimen cause an inaccurate hemoglobin value?
Lipemic specimens contain high levels of triglycerides consisting of chylomicrons and very low-density lipoprotein particles, which in turn cause turbidity. This turbidity interferes with light scatter and the absorption of light, resulting in a false increase of hemoglobin determinations.
What does it mean when a blood sample is lipemic?
Blood is composed of cells and plasma (or serum), a liquid that is normally pale yellow in colour and transparent. Devices that analyze serum specimens (biochemistry tests) monitor three aspect of the serum: lipemia, icterus index and hemolysis index.
What chemistry tests are affected by hemolysis?
Certain lab tests can be affected and the reported results will be inaccurate. It falsely decreases values such as RBC’s, HCT, and aPTT. It can also falsely elevate potassium, ammonia, magnesium, phosphorus, AST, ALT, LDH and PT.
How can lipemic specimens affect CRP results?
This effect could be explained, since lipemia produces an increase in Cp values due to light scattering, as occurs with hemolysis, but when the interference is great enough to raise the absorbance above the range of linearity, the effect is the opposite. Lipemia also modified CRP levels significantly.
How does lipemia affect electrolytes?
Considering 0-350 mg% of triglyceride as the reference, electrolytes concentration mostly decreased over increasing lipemia. Beyond triglyceride concentration of 650mg%, this decline in electrolytes concentration was statistically significantly for samples in all subgroups.
How do you prevent lipemia?
One way to avoid grossly lipemic samples is to ask that patients fast for 12 hours before sample collection. If this is impractical, a mechanical-based means of clot detection should be available when samples are grossly lipemic.
How can lipemic plasma be prevented?
Please avoid food with high fat content before blood donation. If a blood donor consumes food with high fat content such as oily foods which are fried or deep fried before blood donation it can lead to a transient rise in the triglycerides or cholesterol levels resulting in lipemic blood.
Does lipemia affect cholesterol?
These samples were analyzed for total cholesterol (TC) and High Density Lipoprotein-Cholesterol (HDL-C) before and after ultra centrifugation/airfuge. Results: There was a positive correlation between the lipemia and the false high TC and HDL-C.
Which RBC indices are affected by lipemia?
A. Lipemia interferes with the accurate determination of hemoglobin, or Hb, by spectroscopy on most hematology analyzers, but it does not generally interfere with determinations (especially impedance based) of red blood cell count, white blood cell count, and platelet count.
What causes high lipemia?
The most common likely causes of elevated lipemic index were lipid-containing intravenous medications (fat emulsion for parenteral nutrition; propofol) and diabetes mellitus (mainly type 2).
How do you check for clots hemolysis and lipemia on a sample?
Sample collection and handling procedures Collect blood into a plain, red top tube. Allow blood to clot, and then centrifuged to separate serum. Transfer serum to a new plain, red top tube. Samples with marked hemolysis or lipemia should be re-drawn before sample submission.
Does lipemia affect PT and PTT?
Like icterus, these results show that lipemia have no effect on any of PT, aPTT and fibrinogen results.
How does lipemia affect coagulation testing?
Introduction: Lipemia in samples can cause analytical errors in coagulation tests using photometric assays.
Are lipids affected by hemolysis?
Increased Lipid Concentration Is Associated with Increased Hemolysis.
What tests are not affected by hemolysis?
Analytes that were not affected at even the highest level of hemolysis include calcium, chloride, creatinine, C-reactive protein (CRP), glucose and sodium.
What can cause false positive CRP results?
A higher than normal CRP level does not necessarily mean you have a medical condition needing treatment. There are other factors that can raise your CRP levels. These include cigarette smoking, obesity, and lack of exercise. If you have questions about your results, talk to your health care provider.
What does Lipaemic mean?
: the presence of an excess of fats or lipids in the blood specifically : hypercholesterolemia.
What does high lipemia index mean?
Conclusion: A markedly elevated lipemia index in a clear serum sample measured on Siemens analyzers Dimension indicates a high possibility for the presence of a paraprotein in the sample.
What are some reasons a specimen would be lipemic quizlet?
-Lipemia (lactescence) is caused by increased triglycerides (as chylomicrons or very low density lipoproteins). Lipemia is usually a post-prandial artifact (blood collected in a non-fasted animal) and can be minimized by collecting blood from a fasted animal (minimum, 12 hour fast).