Hence, both YAC and BAC are artificial DNA constructs that can maintain large human and plant genomic fragments (> 300 kb) with a high degree of stability and thus, are used to construct genome libraries and in gene transfer. Thus, the correct answer is option D.
What does BAC stand for in bacteria?
What is a BAC? BAC stands for Bacterial Artificial Chromosome. These are small pieces of bacterial? DNA? that can be identified and copied within a bacterial cell. A BAC acts as a vector? to artificially carry DNA into the cell of a bacterium, such as Escherichia coli?, to make a BAC clone?.
How is BAC different from plasmid?
Bacterial Artificial Chromosomes (BAC) have been developed to hold much larger pieces of DNA than a plasmid can. BAC vectors were originally created from part of an unusual plasmid present in some bacteria called the F’ plasmid.
What are BAC vectors?
BAC vectors are plasmids constructed with the replication origin of E. coli F factor, and so can be maintained in a single copy per cell. These vectors can hold DNA fragments of up to 300 kb.
What’s another name for BAC?
What Is Blood Alcohol Concentration (BAC)? Blood Alcohol Concentration (BAC) refers to the percent of alcohol (ethyl alcohol or ethanol) in a person’s blood stream.
Is BAC A expression vector?
The rapid construction of a BAC-based expression vector facilitates heterologous expression of large gene clusters for drug discovery.
What is the difference between BAC and why AC?
The main difference between YAC and BAC vectors is that the YAC vectors (Yeast Artificial Chromosome vectors) contain the molecular components for the replication inside yeast whereas the BAC vectors (Bacterial Artificial Chromosome Vectors) contain molecular components for the replication inside bacteria.
What are the advantages of using BAC in cloning?
The advantages of the BAC vector are that it can maintain up to 300 kb of DNA, and the clones are highly stable in their host even after 100 generations. BACs thus facilitate the construction of DNA libraries of complex genomes because they allow fuller representation of all sequences.
What are YAC and BAC in gene cloning Class 12?
The term YAC stands for yeast artificial chromosome and the term BAC stands for bacterial artificial chromosomes. These are the two vectors which are basic tools to insert the gene of interest in the host cell.
Who invented BAC?
In 1997, the first virus BAC system was developed by Messerle and colleagues for the murine cytomegalovirus (MCMV), one of the largest herpesviruses with a genome size of 230 kbp .
In which of the following technique was BAC and why AC used?
The inserted gene of interest of DNA in BACs is taken by bacterial cells. The bacterial cells amplify DNA as they grow and divide that are then isolated and used in sequencing DNA.
What is a BAC transgenic?
BAC transgenes direct gene expression to specific cell types in tissues more precisely than many promoter mini-constructs. The most rapid and effective method to generate BAC transgenic mice is the direct microinjection of BAC DNA into the pronucleus of fertilized mouse eggs.
What does BAC mean in text?
The abbreviation BAC is typically used in computer-based conversations (such as chat forums and multi-player games) to mean “Back At Computer.” In this context, BAC indicates that a participant who had been away from their computer or console for a brief period has now returned.
How is BAC determined?
Blood alcohol concentration, or BAC, is the metric used to measure the amount of alcohol in a person’s bloodstream at a given time. This alcohol content can be determined by testing the person’s breath, blood, urine, or saliva, with breath testing being the most common method of testing as practiced by law enforcement.
What factors affect your BAC?
- Age. There is no denying that a person’s age can impact how alcohol affects them.
- Tolerance levels. The more a person drinks, the higher their tolerance to alcohol will be.
- Alcohol Concentration.
- Body type.
Is YAC a plasmid?
Construction. A YAC is built using an initial circular DNA plasmid, which is typically cut into a linear DNA molecule using restriction enzymes; DNA ligase is then used to ligate a DNA sequence or gene of interest into the linearized DNA, forming a single large, circular piece of DNA.
What is the full form of BAC and YAC?
YAC stands for Yeast Artificial Chromosome and BAC stands for Bacterial artificial chromosome. YAC are derived from DNA of yeast S. cerevisiae by the process of genetic engineering. They are then ligated into the bacterial plasmid.
What is the main use of a bacterial artificial chromosome BAC vectors Mcq?
7. What is the main use of a Bacterial Artificial Chromosome (BAC) vectors? Explanation: BAC vectors are specifically created to handle longer DNA inserts for the construction of human genomic libraries.
Which gene regulates copy number of BAC in cloned cell?
A gene of interest in a BAC clone is cloned into a plasmid and then modified by recombineering , or BAC DNA is modified in situ by recombineering, and then DNA containing the modified region is cloned into a plasmid vector . Plasmids for targeting constructs are mostly high-copy-number vectors.
What is the maximum size of fragments that can carry by a BAC vector?
BAC :-(Bacterial Artificial Chromosome) vectors, derived from F-plasmid ,can carry inserts ranging from 150-300 kb.
What is YAC BAC in gene cloning Shaalaa?
BAC (bacterial artificial chromosomes) and YAC (yeast artificial chromosomes) are cloning vectors which are used in the Human Genome Project for cloning or amplification of human DNA fragments.
What is BAC biochemistry?
Bacterial artificial chromosomes (BAC) contain large fragments of genomic DNA (150–300kb), and therefore can accommodate entire genes, including the cis-regulatory sequences, allowing for increased fidelity of gene expression (Shizuya et al., 1992).
When was the first breath tester developed and by who?
The first breathalyzer as we currently know it was developed by Robert Frank Borkenstein in 1958. Borkenstein coupled a photometer with a reaction between the alcohol in a subject’s breath and potassium dichromate.
What is the role of BAC and YAC in Human Genome Project?
1 Answer. Detailed answer: YAC (Yeast Artificial Chromosomes) and BAC (Bacterial Artificial chromosomes) are cloning vectors. They are used in Human genome project for cloning or amplification of human DNA fragments.
What do Y and B stand for in YAC and BAC used in the human genome Project and what is its role in it?
UPLOAD PHOTO AND GET THE ANSWER NOW! Solution : (a) The BAC (bacterial artificial Chromosomes and YAC (Yeast artificial chromosomes) represent the host cells used for cloning of DNA fragments using specialized vectors.
(b) Less than 2 percent of the human genome codes for proteins.