What is azan stain used for?

The Azan trichrome stain, sometimes referred to as the Heidenhain’s Azan Trichrome stain is also used to stain muscle and collagen. Therefore, it can be used to differentiate between muscle and collagen tissue, as well as to identify diseases such as liver disorders.

What does Azan Trichrome stain?

Azan Trichrome kit is a modification of Mallory Trichrome kit for staining connective tissue. It is used for visualizing muscles, collagen fibers, glial cells, glomerular cells, chromatins and erythrocytes of the same section. The kit contains two acid dyes: Azocarmine G and Aniline Blue counterstain.

How does H and E staining work?

H&E is the combination of two histological stains: hematoxylin and eosin. The hematoxylin stains cell nuclei a purplish blue, and eosin stains the extracellular matrix and cytoplasm pink, with other structures taking on different shades, hues, and combinations of these colors.

What stains staining techniques are used to demonstrate glycogen?

PAS (periodic acid-Schiff) This an all-around useful stain for many things. It stains glycogen, mucin, mucoprotein, glycoprotein, as well as fungi.

What staining is best for lipid detection?

The Oil Red O (ORO) stain can identify neutral lipids and fatty acids in smears and tissues. Fresh smears or cryostat sections of tissue are necessary because fixatives containing alcohols, or routine tissue processing with clearing, will remove lipids. The ORO is a rapid and simple stain.

Which stain is best for glycogen?

The selective staining of glycogen by Best’s Carmine and the other dyes used in a similar manner (e.g. Alizarin Brilliant Blue BS, Alizarin Red S, Gallein and Haematoxylin) is due to hydrogen bonding between phenolate (−O−) groups of the dye and glycogen hydroxyl groups.

What is Van Gieson stain used for?

Van Gieson’s trichrome is used for the differentiation between collagen and smooth muscle in tumors and to demonstrate the increase in the amount of collagen in these pathologies.

What does Masson’s Trichrome stain?

Masson’s trichrome is a stain that highlights collagen fibers. This method is very popular and is used in histology to differentiate collagen and muscle fibers on tissue sections. It is particularly used in the study of pathologies of the heart (infarction), liver (cirrhosis), kidney (glomerular fibrosis).

What are histological stains?

Histological staining is a series of technique processes undertaken in the preparation of sample tissues by staining using histological stains to aid in the microscope study (Anderson, 2011).

What stains pink in H&E?

What structures are stained pink (eosinophilic or acidophilic)? Most proteins in the cytoplasm are basic, and so eosin binds to these proteins and stains them pink. This includes cytoplasmic filaments in muscle cells, intracellular membranes, and extracellular fibres.

Is hematoxylin basophilic or acidophilic?

Structures called acidophilic are those which normally: stain red or pink with hematoxylin. stain red or pink with eosin.

What is acidophilic and basophilic?

Acidic – Contains acidic groups that have an affinity for basic tissue elements. Basic stains are used to stain nuclei and other basophilic (base-loving) cellular structures in tissues. Acidic stains are used to stain cytoplasm and other acidophilic (acid-loving) cellular structures in tissues.

Why methylene blue is used in staining?

It is a cationic dye that stains cells blue because the positively charged dye is attracted to negatively charged particles such as polyphosphates, DNAs, and RNAs. Specimens collected from patients by swabbing are smeared onto microscope slides and the methylene blue solution is dropped on the slide.

What stains staining techniques are used to demonstrate carbohydrates?

  • Periodic Acid-Schiff (PAS): Diagnostic Applications.
  • Periodic acid-Shiff (PAS): Chemistry.
  • Periodic acid-Shiff (PAS): Staining Protocol.
  • Periodic Acid-Schiff (PAS) with Diastase: Diagnostic Applications.

What are the different types of staining techniques?

  • Types of staining techniques. Simple staining.
  • Differential staining. (Use of of single stain)
  • (Use of two contrasting stains) Direct.
  • Indirect. Separation.
  • Visualization. (Positive)
  • (Negative) into groups. of structures.
  • Gram stain. Flagella stain.
  • Acid fast. Capsule stain.

What are the 3 types of special stains?

  • Massons Trichrome. The trichrome stain helps to highlight the supporting collagenous stroma in sections from a variety of organs.
  • Verhoff’s Elastic Stain.
  • Reticulin Stain.
  • Giemsa Stain.

Which stain is used for amyloid deposits?

Congo Red and Thioflavin S are the two major histological stains used to detect any form of amyloid. These dyes bind to the characteristic β-pleated sheet conformation of amyloid.

Which stain is used for calcium?

With the H&E stain, calcium appear deep blue-purple. PRINCIPLE: Tissue sections are treated with silver nitrate solution, the calcium is reduced by the strong light and replaced with silver deposits, visualized as metallic silver.

How do you test for glycogen?

First, glycogen is digested into glucose using glucoamylase enzyme. The resulting glucose is then measured using glucose dehydrogenase in conjunction with a bioluminescent NADH detection technology. The result is a light signal proportional to the starting glycogen concentration in the sample.

How do you measure glycogen?

Currently the typical method to measure muscle glycogen requires an invasive muscle biopsy. Involving the use of needles, muscle biopsies have been the standard method to measure muscle glycogen. This procedure is common in sports science but does have its drawbacks due to its invasive nature.

How do you demonstrate glycogen?

  1. Place two identical tissue sections – one per slide.
  2. Treat slide A with water or buffer solution.
  3. Treat slide B with diastase (a-amylase 0.25g dissolved in 50 ml distilled water)
  4. Stain both slides with PAS per protocol above.
  5. Dehydrate, clear, and mount.

Is Van Gieson a special stain?

Verhoeff-van Gieson Stain: A Special Histology Stain for Elastic Fibers.

What stain is used for elastic fibers?

The Verhoeff stain is one of the most commonly-used stains to visualize elastic tissue, as found in blood vessel walls, elastic cartilage, lungs, skin, bladder, and some ligaments.

What stains collagen pink?

Haematoxylin stains cell nucleus violet in color. However, cell nuclei may appear black or brown or grey. Under a bright-field microscope, collagen appears pink to red in color on a pale yellow background of cytoplasm as a result of staining with Sirius red dye.

How does trichrome staining highlight collagen?

A second acid dye (blue or green) in dilute acetic acid is applied which, in turn, displaces the polyacid, resulting in collagen stained in a contrasting color to the initial dye used. The stain will demonstrate the presence collagen in the mucosal subepithelial collagen layer of colon tissue.

Do NOT follow this link or you will be banned from the site!