Bovine Serum Albumin (BSA) is used in a variety of laboratory applications including its function as a protein concentration standard, its function as a cell nutrient and its ability to stabilize enzymes during restriction digest.
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What is BSA made from?
Bovine serum albumin structure and biological functions The BSA molecule consists of 583 amino acids, bound in a single chain cross-linked with 17 cystine residues (eight disulfide bonds and one free thiol group), and has a molecular mass of 66400 Da [1].
What does BSA protein stand for?
BSA for various cell culture applications Bovine Serum Albumin, also known as “BSA” or “Fraction V”, is a protein derived from bovine blood plasma. It is a small, stable, and moderately non-reactive protein, and therefore often used as a blocker in immunohistochemistry.
Is BSA a protein?
BSA is a protein found predominantly in the circulatory system of the cow but is also a constituent of the whey component of bovine milk.
Why is BSA used as a blocking agent?
The primary role of BSA is to prevent the non-specific binding by blocking the leftover spaces over solid surface after immobilization of a capture biomolecule.
What is the BSA formula?
Formulas to estimate body surface area (BSA) Boyd* BSA (m2) = 0.0003207 x Ht (cm)0.3 x Wt (g)(0.7285 โ ( 0.0188 x LOG(Wt (g)))) DuBois and DuBois* BSA (m2) = Wt (kg)0.425 x Ht (cm)0.725 x 0.007184.
What is a BSA standard?
Bovine Serum Albumin (BSA) is the standard reference for total protein quantitation by colorimetric assays. Expedeon BSA standards are designed for serial dilution to generate accurate standard curves and are precisely formulated at 2mg/ml.
How does BSA stabilize proteins?
BSA stabilizes Streptococcus thermophilus beta-galactosidase against thermal inactivation and binds to the active enzyme subunits formed on heating. The mechanism of interaction and stabilization, however, is unknown, and it was investigated using different proteins.
How do you make BSA?
Manufacturing Methods Bovine serum albumin (BSA) is purified from serum using one of three different purification methods: cold-organic solvent fractionation, heat shock and ion exchange chromatography.
What is BSA lab test?
A serum albumin test measures the amount of this protein in the clear liquid portion of the blood. Albumin can also be measured in the urine. Blood is drawn from a vein (venipuncture), usually from the inside of the elbow or the back of the hand.
Is BSA a carbohydrate?
From these results we concluded that 1) specific carbohydrates which are probably involved in embryonic development appear much earlier in the embryo than the endogenous lectins which are able to react with these carbohydrates and 2) BSA is a protein which like WGA probably binds N-acetylglucosamine or sialic acid …
What is BSA and why is it used in this protein assay?
BSA is the most commonly used standard for relative protein concentration determination in most laboratories, although the color response of ฮณ-globulin is usually more representative of true concentration for samples that do not have a high albumin content.
Does BSA contain calcium?
Calcium ions that are bound to BSA are shown as black spheres. In the crystal structure of bovine albumin we observe binding of three calcium ions.
Does BSA contain fatty acids?
Due to BSA’s biological role as a fatty acid transporter, BSA proteins contain bound fatty acids that were either naturally bound, or exogenously added as part of the heat-shock fractionation process and can be optionally removed by an extra processing step.
What kind of protein is BSA?
Bovine serum albumin (BSA) is a globular protein (~66 kDa MW) that is used in numerous biochemical applications due to its stability and lack of interference with biological reactions. The BSA structure is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates.
Is BSA a monomer?
Analysis of these data indicated that BSA is in a monomer-dimer equilibrium with a dissociation constant of 10 +/- 2 microM at 25 degrees C in 10 mM MOPS-K (pH 5.8).
Why is bovine serum albumin used?
Bovine Serum Albumin (BSA) is commonly used in cell culture protocols, particularly where protein supplementation is necessary and the other serum components are unwanted. In cell culture, it acts as a small molecule carrier.
What is BSA blocking buffer?
BSA blocking buffer have a high affinity for proteins. A variety of blocking buffers ranging from milk or normal serum to highly purified proteins have been used to block free sites on a membrane. It is a good blocking agent that is easily prepared in the lab. It can be used in a range from 0.3% to 5%.
How does BSA work in PCR?
BSA significantly enhances PCR amplification yield when used in combination with organic solvents, DMSO or formamide. BSA enhancing effects were obtained in several PCR applications, with DNA templates of high GC content and spanning a broad size range.
What is normal BSA range?
The overall BSA was 2.04 +/- 0.24 m(2): 1.81 +/- 0.19 m(2) in normal-weight, 1.99 +/- 0.16 m(2) in overweight, and 2.21 +/- 0.22 m(2) in obese subjects. These values were significantly higher in overweight and obese patients compared with the values using the DuBois-DuBois formula (overall, 2.00 +/- 0.22 m(2), P < .
What BSA is overweight?
The purpose of the present study was to examine the BSA based on Mosteller’s formula in normal-weight (body mass index [BMI], 20-24.9 kg/m2), overweight (BMI, 25-29.9 kg/m2), and obese (BMI, โฅ30 kg/m2) adults (>18 years old) in comparison with other empirically derived formulas (DuBois and DuBois, Boyd [The growth of …
How is BSA calculated in pharmacy?
What is protein quantification used for?
Protein quantification is necessary to understand the total protein content in a sample or in a formulated product. Accurate protein quantification is important as a range of other critical assays require precise total protein content results in order to generate data.
How do you make 10 mg BSA mL?
Weigh 100 mg BSA and dissolve in 10 mL water to make 10 mg/mL BSA solution as stock.
Why is BSA fatty acid free?
Fatty acid-free BSA is a highly useful reagent for such cell culture experiments, to allow researchers to use such BSA as a carrier for their specific fatty acids specific to their cell culture system. This mitigates potential risks to the cells from FAโฒs that might be present in non-FA-free BSA.